Exosomes Facilitate Transmission of Enterovirus A71 From Human Intestinal Epithelial Cells.

ABSTRACT:

Background

Enterovirus A71 (EV-A71) has been noted for its tendency to lead to neurological manifestations in young children and infants. Although the alimentary tract has been identified as the primary replication site of this virus, how EV-A71 replicates in the gut and is transmitted to other organs remains unclear.

Methods

By using differentiated C2BBe1 cells as a model, we observed that intestinal epithelial cells (IECs) were permissive to EV-A71 infection, and viral particles were released in a nonlytic manner.

Results

The coexistence of active caspase 3 and EV-A71 protein was observed in the infected undifferentiated C2BBe1 and RD cells but not in the infected differentiated C2BBe1 cells. Furthermore, EV-A71 infection caused differentiated C2BBe1 and intestinal

SUBMITTER: Huang HI 

PROVIDER: S-EPMC7336570 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature