DLX5 and HOXC8 enhance the chondrogenic differentiation potential of stem cells from apical papilla via LINC01013.

ABSTRACT:

Background

Mesenchymal stem cell (MSC)-based cartilage tissue regeneration is a treatment with great potential. How to enhance the MSC chondrogenic differentiation is a key issue involved in cartilage formation. In the present study, we seek to expound the phenotypes and mechanisms of DLX5 in chondrogenic differentiation function in MSCs.

Methods

Stem cells from apical papilla (SCAPs) were used. The Alcian Blue staining, pellet culture system, and cell transplantation in rabbit knee cartilage defect were used to evaluate the chondrogenic differentiation function of MSCs. Western blot, real-time RT-PCR, and ChIP assays were used to evaluate the molecular mechanisms.

Results

DLX5 and HOXC8 expressions were upregulated during chondrogenic differentiation. In vitro result

SUBMITTER: Yang H 

PROVIDER: S-EPMC7336658 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature