A Light-Inducible Strain for Genome-Wide Histone Turnover Profiling in Neurospora crassa.

ABSTRACT: In chromatin, nucleosomes are composed of ∼146 bp of DNA wrapped around a histone octamer, and are highly dynamic structures subject to remodeling and exchange. Histone turnover has previously been implicated in various processes including the regulation of chromatin accessibility, segregation of chromatin domains, and dilution of histone marks. Histones in different chromatin environments may turnover at different rates, possibly with functional consequences. Neurospora crassa sports a chromatin environment that is more similar to that of higher eukaryotes than yeasts, which have been utilized in the past to explore histone exchange. We constructed a simple light-inducible system to profile histone exchange in N. crassa on a 3xFLAG-tagged histone H3 under the control of the

SUBMITTER: Storck WK 

PROVIDER: S-EPMC7337079 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature