Cellular Force Nanoscopy with 50 nm Resolution Based on Integrin Molecular Tension Imaging and Localization.

ABSTRACT: Integrin-transmitted cellular forces have rich spatial dynamics and are vital to many cellular functions. To advance the sensitivity and spatial resolution of cellular force imaging, we developed a force-activatable emitter reporting single-molecular tension events and the associated cellular force nanoscopy (CFN). Immobilized on a surface, the emitters are initially dark (>99.8% quenched), providing a low fluorescence background despite the high coating density (>2000/μm²) required for sampling cellular force properly. The emitters fluoresce brightly once switched on by integrin tensions and can be switched off by photobleaching, enabling continuous real-time imaging of integrin molecular tensions in live cells. With multiple cycles of molecular tension imaging and localization

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC7337222 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature