Effect of Ionizing Radiation on Transcriptome during Neural Differentiation of Human Embryonic Stem Cells.

ABSTRACT: Human embryonic brain development is highly sensitive to ionizing radiation. However, detailed information on the mechanisms of this sensitivity is not available due to limited experimental data. In this study, differentiation of human embryonic stem cells (hESCs) to neural lineages was used as a model for early embryonic brain development to assess the effect of exposure to low (17 mGy) and high (572 mGy) doses of radiation on gene expression. Transcriptomes were assessed using RNA sequencing during neural differentiation at three time points in control and irradiated samples. The first time point was when the cells were still pluripotent (day 0), the second time point was during the stage of embryoid body formation (day 6), and the third and final time point was during the stage of neura

SUBMITTER: Loeliger BW 

PROVIDER: S-EPMC7337254 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature