Novel Carbapenem-Resistant Klebsiella pneumoniae ST147 Coharboring bla _NDM-1, bla _OXA-48 and Extended-Spectrum β-Lactamases from Pakistan.

ABSTRACT:

Purpose

The emergence of multidrug-resistant Klebsiella pneumoniae (K. pneumoniae) is associated with the acquisition of multiple carbapenemases. Their clonal spread is a worldwide concern due to their critical role in nosocomial infections. Therefore, the identification of high-risk clones with antibiotic resistance genes is very crucial for controlling its global spread.

Materials and methods

A total of 227 K. pneumoniae strains collected during April 2018 to November 2019 were confirmed by PCR. Carbapenemases and extended-spectrum β-lactamases (ESBL) were detected phenotypically. Confirmation of carbapenemases was carried out by PCR and Sanger sequencing. The clonal lineages were assigned to selected isolates by multilocus sequence typing (MLST), and the pla

SUBMITTER: Gondal AJ 

PROVIDER: S-EPMC7337428 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature