A Cross-linking Mass Spectrometry Approach Defines Protein Interactions in Yeast Mitochondria.

ABSTRACT: Protein cross-linking and the analysis of cross-linked peptides by mass spectrometry is currently receiving much attention. Not only is this approach applied to isolated complexes to provide information about spatial arrangements of proteins, but it is also increasingly applied to entire cells and their organelles. As in quantitative proteomics, the application of isotopic labeling further makes it possible to monitor quantitative changes in the protein-protein interactions between different states of a system. Here, we cross-linked mitochondria from Saccharomyces cerevisiae grown on either glycerol- or glucose-containing medium to monitor protein-protein interactions under non-fermentative and fermentative conditions. We investigated qualitatively the protein-protein interactions o

SUBMITTER: Linden A 

PROVIDER: S-EPMC7338081 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature