Third Harmonic Generation microscopy distinguishes malignant cell grade in human breast tissue biopsies.

ABSTRACT: The ability to distinguish and grade malignant cells during surgical procedures in a fast, non-invasive and staining-free manner is of high importance in tumor management. To this extend, Third Harmonic Generation (THG), Second Harmonic Generation (SHG) and Fourier-Transform Infrared (FTIR) spectroscopy were applied to discriminate malignant from healthy cells in human breast tissue biopsies. Indeed, integration of non-linear processes into a single, unified microscopy platform offered complementary structural information within individual cells at the submicron level. Using a single laser beam, label-free THG imaging techniques provided important morphological information as to the mean nuclear and cytoplasmic area, cell volume and tissue intensity, which upon quantification could not onl

SUBMITTER: Gavgiotaki E 

PROVIDER: S-EPMC7338369 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature