Development of simple and efficient Lab-on-a-Disc platforms for automated chemical cell lysis.

ABSTRACT: Cell lysis is the most important first step for molecular biology and diagnostic testing. Recently, microfluidic systems have attracted considerable attention due to advantages associated with automation, integration and miniaturization, especially in resource-limited settings. In this work, novel centrifugal microfluidic platforms with new configurations for chemical cell lysis are presented. The developed systems employ passive form of pneumatic and inertial forces for effective mixing of lysis reagents and cell samples as well as precise fluidic control. Characterizations of the developed Lab-on-a-Discs (LoaDs) have been conducted with dyed deionized (DI) waters and white blood cells (WBCs) to demonstrate the suitability of the proposed systems in terms of mixing, fluidic control and ch

SUBMITTER: Jahromi AK 

PROVIDER: S-EPMC7338454 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature