Efficient Multiplex Genome Editing in Streptomyces via Engineered CRISPR-Cas12a Systems.

ABSTRACT: Streptomyces strains produce a great number of valuable natural products. With the development of genome sequencing, a vast number of biosynthetic gene clusters with high potential for use in the discovery of valuable clinical drugs have been revealed. Therefore, emerging needs for tools to manipulate these biosynthetic pathways are presented. Although the clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas 9) system has exhibited great capabilities for gene editing in multiple Streptomyces strains, it has failed to work in some newly discovered strains and some important industrial strains. Additionally, the protospacer adjacent motif (PAM) recognition scope of this system sometimes limits its applications for generating precise

SUBMITTER: Zhang J 

PROVIDER: S-EPMC7338789 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature