Selecting optimal spectral bands for improved detection of autofluorescent biomarkers in multiphoton microscopy.

ABSTRACT:

Significance

In multiphoton microscopy, two-photon excited fluorescence (TPEF) spectra carry valuable information on morphological and functional biological features. For measuring these biomarkers, separation of different parts of the fluorescence spectrum into channels is typically achieved by the use of optical band pass filters. However, spectra from different biomarkers can be unknown or overlapping, creating a crosstalk in between the channels. Previously, establishing these channels relied on prior knowledge or heuristic testing.

Aim

The presented method aims to provide spectral bands with optimal separation between groups of specimens expressing different biomarkers.

Approach

We have developed a system capable of resolving TPEF with high spectral resolution fo

SUBMITTER: Meyer BO 

PROVIDER: S-EPMC7338838 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature