P75NTR optimizes the osteogenic potential of human periodontal ligament stem cells by up-regulating α1 integrin expression.

ABSTRACT: Human periodontal ligament stem cells (hPDLSCs) are a promising source in regenerative medicine. Due to the complexity and heterogeneity of hPDLSCs, it is critical to isolate homogeneous hPDLSCs with high regenerative potential. In this study, p75 neurotrophin receptor (p75NTR) was used to isolate p75NTR⁺ and p75NTR^- hPDLSCs by fluorescence-activated cell sorting. Differences in osteogenic differentiation among p75NTR⁺ , p75NTR^- and unsorted hPDLSCs were observed. Differential gene expression profiles between p75NTR⁺ and p75NTR^- hPDLSCs were analysed by RNA sequencing. α1 Integrin (ITGA1) small interfering RNA and ITGA1-overexpressing adenovirus were used to transfect p75NTR⁺ and p75NTR^- hPDLSCs. The re

SUBMITTER: Li J 

PROVIDER: S-EPMC7339167 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature