Development of a high-throughput arrayed neural circuitry platform using human induced neurons for drug screening applications.

ABSTRACT: Proper brain function relies on the precise arrangement and flow of information between diverse neural subtypes. Developing improved human cell-based models which faithfully mimic biologically relevant connectivity patterns may improve drug screening efforts given the limited success of animal models to predict safety and efficacy of therapeutics in human clinical trials. To address this need, we have developed experimental models of defined neural circuitries through the compartmentalization of neuronal cell subtypes in a 96 well plate-based platform where each microwell is divided into two compartments connected by microchannels allowing high-throughput screening (HTS) of small molecules. We demonstrate that we can generate subtype-specific excitatory and inhibitory induced neuronal cell

SUBMITTER: Fantuzzo JA 

PROVIDER: S-EPMC7339603 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature