Comprehensive investigation of T and B cell receptor repertoires in an MC38 tumor model following murine anti‑PD‑1 administration.

ABSTRACT: The MC38 (derived from carcinogen‑induced colon adenocarcinoma) tumor model is sensitive to anti‑programmed cell death‑1 (anti-PD‑1) treatment. However, there is no comprehensive description of the T and B cell receptor (TCR, BCR) repertoires of the MC38 tumor model following anti‑PD‑1 treatment, an improved understanding of which is highly important in the development of anti‑PD‑1 immunotherapy. The present study analyzed the TCR and BCR repertoires of three types of tissue, including tumor, spleen and tumor draining lymph node (DLN) from 20 MC38 syngeneic mice receiving murine anti‑PD‑1 (mDX400) treatment or mouse immunoglobulin G1 (mIgG1) control treatment. To obtain enough tissues for high‑throughput sequencing, samples were collected on day 8 after the start of initial treatment. The

SUBMITTER: Zhang L 

PROVIDER: S-EPMC7339640 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature