Long non‑coding RNA NNT‑AS1 knockdown represses the progression of gastric cancer via modulating the miR‑142‑5p/SOX4/Wnt/β‑catenin signaling pathway.

ABSTRACT: Patients with advanced gastric cancer (GC) have a poor prognosis with a median overall survival of 10‑12 months. Long non‑coding RNA nicotinamide nucleotide transhydrogenase‑antisense RNA1 (NNT‑AS1) and sex‑determining region Y‑related high mobility group box 4 (SOX4) have been reported to be associated with the progression of various types of cancer; however, the regulatory mechanism between NNT‑AS1 and SOX4 in GC is not completely understood. Reverse transcription‑quantitative PCR was used to detect the expression levels of NNT‑AS1, microRNA (miR)‑142‑5p and SOX4. Western blotting was performed to assess the protein expression levels of SOX4, β‑catenin, c‑Myc, Bcl‑2 and E‑cadherin. The proliferation, apoptosis, migration and invasion of GC cells were determined using MTT, flow cytometry

SUBMITTER: Zhang J 

PROVIDER: S-EPMC7339722 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature