Urethral luminal epithelia are castration-insensitive cells of the proximal prostate.

ABSTRACT:

Background

Castration-insensitive epithelial progenitors capable of regenerating the prostate have been proposed to be concentrated in the proximal region based on facultative assays. Functional characterization of prostate epithelial populations isolated with individual cell surface markers has failed to provide a consensus on the anatomical and transcriptional identity of proximal prostate progenitors.

Methods

Here, we use single-cell RNA sequencing to obtain a complete transcriptomic profile of all epithelial cells in the mouse prostate and urethra to objectively identify cellular subtypes. Pan-transcriptomic comparison to human prostate cell types identified a mouse equivalent of human urethral luminal cells, which highly expressed putative prostate progenitor markers. V

SUBMITTER: Joseph DB 

PROVIDER: S-EPMC7339731 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature