A gene expression signature for chemoradiosensitivity of colorectal cancer cells.

ABSTRACT:

Purpose

The standard treatment of patients with locally advanced rectal cancers comprises preoperative 5-fluorouracil-based chemoradiotherapy followed by standardized surgery. However, tumor response to multimodal treatment has varied greatly, ranging from complete resistance to complete pathologic regression. The prediction of the response is, therefore, an important clinical need.

Methods and materials

To establish in vitro models for studying the molecular basis of this heterogeneous tumor response, we exposed 12 colorectal cancer cell lines to 3 μM of 5-fluorouracil and 2 Gy of radiation. The differences in treatment sensitivity were then correlated with the pretherapeutic gene expression profiles of these cell lines.

Results

We observed a heterogeneous response,

SUBMITTER: Spitzner M 

PROVIDER: S-EPMC7339824 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature