The role of extracellular vesicles and PD-L1 in glioblastoma-mediated immunosuppressive monocyte induction.

ABSTRACT:

Background

Immunosuppression in glioblastoma (GBM) is an obstacle to effective immunotherapy. GBM-derived immunosuppressive monocytes are central to this. Programmed cell death ligand 1 (PD-L1) is an immune checkpoint molecule, expressed by GBM cells and GBM extracellular vesicles (EVs). We sought to determine the role of EV-associated PD-L1 in the formation of immunosuppressive monocytes.

Methods

Monocytes collected from healthy donors were conditioned with GBM-derived EVs to induce the formation of immunosuppressive monocytes, which were quantified via flow cytometry. Donor-matched T cells were subsequently co-cultured with EV-conditioned monocytes in order to assess effects on T-cell proliferation. PD-L1 constitutive overexpression or short hairpin RNA-mediated knockdown

SUBMITTER: Himes BT 

PROVIDER: S-EPMC7339906 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature