Millisecond time-resolved serial oscillation crystallography of a blue-light photoreceptor at a synchrotron.

ABSTRACT: The recent development of serial crystallography has popularized time-resolved crystallography as a technique to determine the structure of protein-reaction intermediate states. However, most approaches rely on the availability of thousands to millions of microcrystals. A method is reported here, using monochromatic synchrotron radiation, for the room-temperature collection, processing and merging of X-ray oscillation diffraction data from <100 samples in order to observe the build up of a photoreaction intermediate species. Using this method, we monitored with a time resolution of 63 ms how the population of a blue-light photoreceptor domain in a crystal progressively photoconverts from the dark to the light state. The series of resulting snapshots allows us to visualize in detail the gra

SUBMITTER: Aumonier S 

PROVIDER: S-EPMC7340269 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature