1.8 A resolution structure of β-galactosidase with a 200 kV CRYO ARM electron microscope.

ABSTRACT: We report the determination of the structure of Escherichia coli β-galactosidase at a resolution of ∼1.8 Å using data collected on a 200 kV CRYO ARM microscope equipped with a K3 direct electron detector. The data were collected in a single 24 h session by recording images from an array of 7 × 7 holes at each stage position using the automated data collection program SerialEM. In addition to the expected features such as holes in the densities of aromatic residues, the map also shows density bumps corresponding to the locations of hydrogen atoms. The hydrogen densities are useful in assigning absolute orientations for residues such as glutamine or asparagine by removing the uncertainty in the fitting of the amide groups, and are likely to be especially relevant in the context

SUBMITTER: Merk A 

PROVIDER: S-EPMC7340270 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature