The Wg and Dpp morphogens regulate gene expression by modulating the frequency of transcriptional bursts.

ABSTRACT: Morphogen signaling contributes to the patterned spatiotemporal expression of genes during development. One mode of regulation of signaling-responsive genes is at the level of transcription. Single-cell quantitative studies of transcription have revealed that transcription occurs intermittently, in bursts. Although the effects of many gene regulatory mechanisms on transcriptional bursting have been studied, it remains unclear how morphogen gradients affect this dynamic property of downstream genes. Here we have adapted single molecule fluorescence in situ hybridization (smFISH) for use in the Drosophila wing imaginal disc in order to measure nascent and mature mRNA of genes downstream of the Wg and Dpp morphogen gradients. We compared our experimental results with predictions from stochast

SUBMITTER: Bakker R 

PROVIDER: S-EPMC7340504 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature