Molecular resolution imaging by post-labeling expansion single-molecule localization microscopy (Ex-SMLM).

ABSTRACT: Expansion microscopy (ExM) enables super-resolution fluorescence imaging of physically expanded biological samples with conventional microscopes. By combining ExM with single-molecule localization microscopy (SMLM) it is potentially possible to approach the resolution of electron microscopy. However, current attempts to combine both methods remained challenging because of protein and fluorophore loss during digestion or denaturation, gelation, and the incompatibility of expanded polyelectrolyte hydrogels with photoswitching buffers. Here we show that re-embedding of expanded hydrogels enables dSTORM imaging of expanded samples and demonstrate that post-labeling ExM resolves the current limitations of super-resolution microscopy. Using microtubules as a reference structure and centrioles, w

SUBMITTER: Zwettler FU 

PROVIDER: S-EPMC7340794 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature