Electron Microscopy for 3D Scaffolds-Cell Biointerface Characterization.

ABSTRACT: Cell fate is largely determined by interactions that occur at the interface between cells and their surrounding microenvironment. For this reason, especially in the field of tissue-engineering, there is a growing interest in developing techniques that allow evaluating cell-material interaction at the nanoscale, particularly focusing on cell adhesion processes. While for 2D culturing systems a consolidated series of tools already satisfy this need, in 3D environments, more closely recapitulating complex in vivo structures, there is still a lack of procedures furthering the comprehension of cell-material interactions. Here, the use of scanning electron microscopy coupled with a focused ion beam (SEM/FIB) for the characterization of cell interactions with 3D scaffolds obtained by different fa

SUBMITTER: Iandolo D 

PROVIDER: S-EPMC7340848 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature