Epigenome-450K-wide methylation signatures of active cigarette smoking: The Young Finns Study.

ABSTRACT: Smoking as a major risk factor for morbidity affects numerous regulatory systems of the human body including DNA methylation. Most of the previous studies with genome-wide methylation data are based on conventional association analysis and earliest threshold-based gene set analysis that lacks sensitivity to be able to reveal all the relevant effects of smoking. The aim of the present study was to investigate the impact of active smoking on DNA methylation at three biological levels: 5'-C-phosphate-G-3' (CpG) sites, genes and functionally related genes (gene sets). Gene set analysis was done with mGSZ, a modern threshold-free method previously developed by us that utilizes all the genes in the experiment and their differential methylation scores. Application of such method in DNA methylatio

SUBMITTER: Mishra PP 

PROVIDER: S-EPMC7340865 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature