Differential Monocyte Actuation in a Three-Organ Functional Innate Immune System-on-a-Chip.

ABSTRACT: A functional, human, multiorgan, pumpless, immune system-on-a-chip featuring recirculating THP-1 immune cells with cardiomyocytes, skeletal muscle, and liver in separate compartments in a serum-free medium is developed. This in vitro platform can emulate both a targeted immune response to tissue-specific damage, and holistic proinflammatory immune response to proinflammatory compound exposure. The targeted response features fluorescently labeled THP-1 monocytes selectively infiltrating into an amiodarone-damaged cardiac module and changes in contractile force measurements without immune-activated damage to the other organ modules. In contrast to the targeted immune response, general proinflammatory treatment of immune human-on-a-chip systems with lipopolysaccharide (LPS) and interferon-

SUBMITTER: Sasserath T 

PROVIDER: S-EPMC7341107 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature