Robot technology identifies a Parkinsonian therapeutics repurpose to target stem cells of glioblastoma.

ABSTRACT: Aim: Glioblastoma is a heterogeneous lethal disease, regulated by a stem-cell hierarchy and the neurotransmitter microenvironment. The identification of chemotherapies targeting individual cancer stem cells is a clinical need. Methodology: A robotic workstation was programmed to perform a drug concentration to cell-growth analysis on an in vitro model of glioblastoma stem cells (GSCs). Mode-of-action analysis of the selected top substance was performed with manual repetition assays and acquisition of further parameters. Results: We identified 22 therapeutic potential substances. Three suggested a repurpose potential of neurotransmitter signal-modulating agents to target GSCs, out of which the Parkinson's therapeutic trihexyphenidyl was most effective. Manual rep

SUBMITTER: Vargas-Toscano A 

PROVIDER: S-EPMC7341159 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature