Characterization of Endolysin LyJH307 with Antimicrobial Activity Against Streptococcus Bovis.

ABSTRACT: Streptococcusbovis (S.bovis) is one of the critical initiators of acute acidosis in ruminants. Therefore, we aimed to develop and characterize the endolysin LyJH307, which can lyse ruminal S.bovis. We tested the bactericidal activity of recombinant LyJH307 against S. bovis JB1 under a range of pH, temperature, NaCl, and metal ion concentrations. In silico analyses showed that LyJH307 has a modular design with a distinct, enzymatically active domain of the NLPC/P60 superfamily at the N-terminal and a cell wall binding domain of the Zoocin A target recognition domain (Zoocin A_TRD) superfamily at the C-terminal. The lytic activity of LyJH307 against S.bovis JB1 was the highest at pH 5.5, and relatively higher under acidic, than under alkaline conditions. LyJH307 activity was also the highest

SUBMITTER: Kim H 

PROVIDER: S-EPMC7341270 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature