Dataset Information

Metataxonomic and Histopathological Study of Rabbit Epizootic Enteropathy in Mexico.

ABSTRACT: Epizootic rabbit enteropathy (ERE) affects young rabbits and represents 32% of the enteropathies in rabbit production farms in Mexico. The etiology of this syndrome has not been clarified yet. A metataxonomic and histopathology study of ERE was carried out to compare the gastrointestinal microbiota and histopathological lesions of healthy and positive-ERE rabbits. The metataxonomic study was done using an Illumina MiSeq (MiSeq® system, Illumina, San Diego California, USA) massive segmentation platform, and a Divisive Amplicon Denoising Algorithm 2 (DADA2 algorithm) was used to obtain Shannon and Simpson diversity indices as well as the relative abundance of the identified communities. For the histopathological study, paraffin sections of the cecum, ileo-cecal valve, and colon were stained with eosin and hematoxylin. AxioVision 4.9 software (Carl Zeiss MicroImaging GmbH, Jena, Germany) was used to measure the crypt depths. Statistical analysis was done using PERMANOVA analysis for the metataxonomic study and ANOVA for the histopathology study. Histopathologic analysis showed smaller sizes of crypts in the colon of ERE rabbits. Differences were observed in the diversity and abundance of the gastrointestinal microbiota between the analyzed groups. The genus Clostridium and the species Cloacibacillus porcorum and Akkermansia muciniphila were associated with ERE. The results obtained from this study can provide information for future clarification of the etiology and proposals of effective treatments.

SUBMITTER: Puon-Pelaez XD

PROVIDER: S-EPMC7341505 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature

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Metataxonomic and Histopathological Study of Rabbit Epizootic Enteropathy in Mexico.

Puón-Peláez Xiao-Haitzi Daniel XD McEwan Neil Ross NR Gómez-Soto José Guadalupe JG Álvarez-Martínez Roberto Carlos RC Olvera-Ramírez Andrea Margarita AM

Animals : an open access journal from MDPI 20200528 6

Epizootic rabbit enteropathy (ERE) affects young rabbits and represents 32% of the enteropathies in rabbit production farms in Mexico. The etiology of this syndrome has not been clarified yet. A metataxonomic and histopathology study of ERE was carried out to compare the gastrointestinal microbiota and histopathological lesions of healthy and positive-ERE rabbits. The metataxonomic study was done using an Illumina MiSeq (MiSeq<sup>®</sup> system, Illumina, San Diego California, USA) massive segm ...[more]

PMID: 32481706

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Dataset Information

Metataxonomic and Histopathological Study of Rabbit Epizootic Enteropathy in Mexico.

Publications

Metataxonomic and Histopathological Study of Rabbit Epizootic Enteropathy in Mexico.

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