Stress-induced NLRP3 inflammasome activation negatively regulates fear memory in mice.

ABSTRACT:

Background

Persistent inflammation dysregulation and cognitive decline have been associated with several trauma- and stress-related disorders such as posttraumatic stress disorder (PTSD) and anxiety disorder. Despite the abundant discoveries of neuroinflammation in such disorders, the underlying mechanisms still remain unclear.

Method

Wild-type and Nlrp3^-/- mice were exposed to the electric foot shocks in the contextual fear memory paradigm. Three hours after the electric foot shocks, activation of the NLRP3 inflammasome was investigated through immunoblotting and ELISA. Microglia were isolated and analyzed by quantitative real-time PCR. Hippocampal tissues were collected 3 h and 72 h after the electric foot shocks and subjected to RNA sequencing. MCC950 was admin

SUBMITTER: Dong Y 

PROVIDER: S-EPMC7341659 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature