Comparison of methods and characterization of small RNAs from plasma extracellular vesicles of HIV/HCV coinfected patients.

ABSTRACT: Hepatitis C virus (HCV) and human immunodeficiency virus (HIV) hijack the host exosomal machinery as an additional mechanism of infection and evasion of the immune system, modifying the small RNA (smRNA) cargo during infection. We characterized the surface epitopes of extracellular vesicles (EVs) from plasma HIV/HCV-coinfected patients and their smRNA cargo profile, by comparing different isolation procedures. Six EVs isolation procedures were compared: ultracentrifugation, and five different polyethylene glycol-based methods (commercial, combined with a column purification step and two custom); and two RNA commercial kits (phenol and non-phenol based) were used. High-throughput sequencing of smRNAs was performed. Exosomal surface epitopes were analyzed by the MACSPlex Exosome Kit. Four mi

SUBMITTER: Martinez-Gonzalez E 

PROVIDER: S-EPMC7341746 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature