Imaging flow cytometry methods for quantitative analysis of label-free crystalline silica particle interactions with immune cells.

ABSTRACT: Exposure to respirable fractions of crystalline silica quartz dust particles is associated with silicosis, cancer and the development of autoimmune conditions. Early cellular interactions are not well understood, partly due to a lack of suitable technological methods. Improved techniques are needed to better quantify and study high-level respirable crystalline silica exposure in human populations. Techniques that can be applied to complex biological matrices are pivotal to understanding particle-cell interactions and the impact of particles within real, biologically complex environments. In this study, we investigated whether imaging flow cytometry could be used to assess the interactions between cells and crystalline silica when present within complex biological matrices. Using the respir

SUBMITTER: Vis B 

PROVIDER: S-EPMC7343534 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature