Improving the genome editing efficiency of CRISPR/Cas9 in Arabidopsis and Medicago truncatula.

ABSTRACT:

Main conclusion

An improved CRISPR/Cas9 system with the Arabidopsis UBQ10 promoter-driven Cas9 exhibits consistently high mutation efficiency in Arabidopsis and M. truncatula. CRISPR/Cas9 is a powerful genome editing technology that has been applied in several crop species for trait improvement due to its simplicity, versatility, and specificity. However, the mutation efficiency of CRISPR/Cas9 in Arabidopsis and M. truncatula (Mt) is still challenging and inconsistent. To analyze the functionality of the CRISPR/Cas9 system in two model dicot species, four different promoter-driven Cas9 systems to target phytoene desaturase (PDS) genes were designed. Agrobacterium-mediated transformation was used for the delivery of constructed vectors to host plants. Phenotypic and genotypic analys

SUBMITTER: Wolabu TW 

PROVIDER: S-EPMC7343739 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature