Near-chromosome level genome assembly of the fruit pest Drosophila suzukii using long-read sequencing.

ABSTRACT: Over the past decade, the spotted wing Drosophila, Drosophila suzukii, has invaded Europe and America and has become a major agricultural pest in these areas, thereby prompting intense research activities to better understand its biology. Two draft genome assemblies already exist for this species but contain pervasive assembly errors and are highly fragmented, which limits their values. Our purpose here was to improve the assembly of the D. suzukii genome and to annotate it in a way that facilitates comparisons with D. melanogaster. For this, we generated PacBio long-read sequencing data and assembled a novel, high-quality D. suzukii genome assembly. It is one of the largest Drosophila genomes, notably because of the expansion of its repeatome. We found that despite 16 rounds of full-sib c

SUBMITTER: Paris M 

PROVIDER: S-EPMC7343843 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature