Caspase-3 Activation Correlates With the Initial Mitochondrial Membrane Depolarization in Neonatal Cerebellar Granule Neurons.

ABSTRACT: In this study we evaluated the effect of the reduction in the endoplasmic reticulum calcium concentration ([Ca²⁺]_ER), changes in the cytoplasmic calcium concentration ([Ca²⁺]_i), alteration of the mitochondrial membrane potential, and the ER stress in the activation of caspase-3 in neonatal cerebellar granule cells (CGN). The cells were loaded with Fura-2 to detect changes in the [Ca²⁺]_i and with Mag-fluo-4 to measure variations in the [Ca²⁺]_ER or with TMRE to follow modifications in the mitochondrial membrane potential in response to five different inducers of CGN cell death. These inducers were staurosporine, thapsigargin, tunicamycin, nifedipine and plasma membrane repolarization by switching culture

SUBMITTER: Benitez-Rangel E 

PROVIDER: S-EPMC7343937 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature