Isolation and characterization of WUPyV in polarized human airway epithelial cells.

ABSTRACT:

Background

Washington University polyomavirus (WUPyV) is a novel human polyomavirus detected in childwith acute respiratory infection in 2007. However, the relationship between WUPyV and respiratory diseases has yet to be established for lacking of a suitable in vitro culture system.

Methods

To isolate WUPyV with human airway epithelial (HAE) cells, the positive samples were incubated in HAE, and then the nucleic acid, VP1 protein and virions were detected using real-time PCR, immunofluorescence and electron microscopy respectively.

Results

The result showed that WUPyV could replicate effectively in HAE cells and virions with typical polyomavirus characteristics could be observed. Additionally, the entire genome sequence of the isolated strain (BJ0771) was obtained an

SUBMITTER: Wang C 

PROVIDER: S-EPMC7344044 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature