MicroRNA-221 Modulates Airway Remodeling via the PI3K/AKT Pathway in OVA-Induced Chronic Murine Asthma.

ABSTRACT:

Background

Airway remodeling is one of the most important pathological features of chronic asthma. This study aimed to determine whether microRNA-221 (hereafter, miR-221) can affect airway remodeling in a mouse model of ovalbumin (OVA)-induced chronic asthma.

Methods

Adeno-associated viruses (AAVs) "Bearing miR-221 sponges" were used to downregulate miR-221 in asthmatic mice. Staining with hematoxylin and eosin (H&E), Masson trichrome, and periodic acid-Schiff reagent was used to assess histological changes. The affected signaling pathway in mouse airway smooth muscle cells (ASMCs) was also identified by gene chip technology. A PI3K/AKT-inhibitor (LY294002) was used to confirm the role of the pathway in ASMCs.

Results

The inhibition of miR-221 in a murine asthma model

SUBMITTER: Pan J 

PROVIDER: S-EPMC7344209 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature