Absolute Quantification of Transcription Factors Reveals Principles of Gene Regulation in Erythropoiesis.

ABSTRACT: Dynamic cellular processes such as differentiation are driven by changes in the abundances of transcription factors (TFs). However, despite years of studies, our knowledge about the protein copy number of TFs in the nucleus is limited. Here, by determining the absolute abundances of 103 TFs and co-factors during the course of human erythropoiesis, we provide a dynamic and quantitative scale for TFs in the nucleus. Furthermore, we establish the first gene regulatory network of cell fate commitment that integrates temporal protein stoichiometry data with mRNA measurements. The model revealed quantitative imbalances in TFs' cross-antagonistic relationships that underlie lineage determination. Finally, we made the surprising discovery that, in the nucleus, co-repressors are dramatically more a

SUBMITTER: Gillespie MA 

PROVIDER: S-EPMC7344268 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature