An Optimized in situ Quantification Method of Leaf H₂O₂ Unveils Interaction Dynamics of Pathogenic and Beneficial Bacteria in Wheat.

ABSTRACT: Hydrogen peroxide (H₂O₂) functions as an important signaling molecule in plants during biotic interactions. However, the extent to which H₂O₂ accumulates during these interactions and its implications in the development of disease symptoms is unclear. In this work, we provide a step-by-step optimized protocol for in situ quantification of relative H₂O₂ concentrations in wheat leaves infected with the pathogenic bacterium Pseudomonas syringae pv. atrofaciens (Psa), either alone or in the presence of the beneficial bacterium Herbaspirillum seropedicae (RAM10). This protocol involved the use of 3-3'diaminobenzidine (DAB) staining method combined with image processing to conduct deconvolution a

SUBMITTER: Carril P 

PROVIDER: S-EPMC7344315 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature