The Basis for Natural Multiresistance to Phage in Pseudomonas aeruginosa.

ABSTRACT: Pseudomonas aeruginosa is responsible for long-term infections and is particularly resistant to treatments when hiding inside the extracellular matrix or biofilms. Phage therapy might represent an alternative to antibiotic treatment, but up to 10% of clinical strains appear to resist multiple phages. We investigated the characteristics of P. aeruginosa clinical strains naturally resistant to phages and compared them to highly susceptible strains. The phage-resistant strains were defective in lipopolysaccharide (LPS) biosynthesis, were nonmotile and displayed an important degree of autolysis, releasing phages and pyocins. Complete genome sequencing of three resistant strains showed the existence of a large accessory genome made of multiple insertion elements, genomic islands,

SUBMITTER: Pourcel C 

PROVIDER: S-EPMC7344871 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature