Programmable µChopper Device with On-Chip Droplet Mergers for Continuous Assay Calibration.

ABSTRACT: While droplet-based microfluidics is a powerful technique with transformative applications, most devices are passively operated and thus have limited real-time control over droplet contents. In this report, an automated droplet-based microfluidic device with pneumatic pumps and salt water electrodes was developed to generate and coalesce up to six aqueous-in-oil droplets (2.77 nL each). Custom control software combined six droplets drawn from any of four inlet reservoirs. Using our μChopper method for lock-in fluorescence detection, we first accomplished continuous linear calibration and quantified an unknown sample. Analyte-independent signal drifts and even an abrupt decrease in excitation light intensity were corrected in real-time. The system was then validated with homogeneous insulin

SUBMITTER: Shi N 

PROVIDER: S-EPMC7344876 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature