Highly Localized Enrichment of Trypanosoma brucei Parasites Using Dielectrophoresis.

ABSTRACT: Human African trypanosomiasis (HAT), also known as sleeping sickness, is a vector-borne neglected tropical disease endemic to rural sub-Saharan Africa. Current methods of early detection in the affected rural communities generally begin with general screening using the card agglutination test for trypanosomiasis (CATT), a serological test. However, the gold standard for confirmation of trypanosomiasis remains the direct observation of the causative parasite, Trypanosoma brucei. Here, we present the use of dielectrophoresis (DEP) to enrich T. brucei parasites in specific locations to facilitate their identification in a future diagnostic assay. DEP refers to physical movement that can be selectively induced on the parasites when exposing them to electric field gradients of specific magnitud

SUBMITTER: Keck D 

PROVIDER: S-EPMC7344920 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature