Micromirror-Embedded Coverslip Assembly for Bidirectional Microscopic Imaging.

ABSTRACT: 3D imaging of a biological sample provides information about cellular and subcellular structures that are important in cell biology and related diseases. However, most 3D imaging systems, such as confocal and tomographic microscopy systems, are complex and expensive. Here, we developed a quasi-3D imaging tool that is compatible with most conventional microscopes by integrating micromirrors and microchannel structures on coverslips to provide bidirectional imaging. Microfabricated micromirrors had a precisely 45° reflection angle and optically clean reflective surfaces with high reflectance over 95%. The micromirrors were embedded on coverslips that could be assembled as a microchannel structure. We demonstrated that this simple disposable device allows a conventional microscope to perform

SUBMITTER: Lee D 

PROVIDER: S-EPMC7345240 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature