Development of a 3-D Physical Dynamics Monitoring System Using OCM with DVC for Quantification of Sprouting Endothelial Cells Interacting with a Collagen Matrix.

ABSTRACT: The extracellular matrix (ECM) plays a key role during cell migration, proliferation, and differentiation by providing adhesion sites and serving as a physical scaffold. Elucidating the interaction between the cell and ECM can reveal the underlying mechanisms of cellular behavior that are currently unclear. Analysis of the deformation of the ECM due to cell-matrix interactions requires microscopic, three-dimensional (3-D) imaging methods, such as confocal microscopy and second-harmonic generation microscopy, which are currently limited by phototoxicity and bleaching as a result of the point-scanning approach. In this study, we suggest the use of optical coherence microscopy (OCM) as a live-cell, volumetric, fast imaging tool for analyzing the deformation of fibrous ECM. We optimized such O

SUBMITTER: Kang YG 

PROVIDER: S-EPMC7345655 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature