Tongue microbiome of smokeless tobacco users.

ABSTRACT:

Background

The possibility that smokeless tobacco may contribute to oral carcinogenesis by influencing the oral microbiome has not been explored. This preliminary cross-sectional study sought to assess the effect of using shammah, a form of smokeless tobacco prevalent in Arabia, on the tongue microbiome. Tongue scarping samples were obtained from 29 shammah users (SU; 27.34 ± 6.9 years) and 23 shammah non-users (SNU; 27.7 ± 7.19 years) and analyzed with 16S rRNA gene sequencing (V1-V3). Species-level taxonomy assignment of the high-quality, merged reads was obtained using a previously described BLASTn-based algorithm. Downstream analyses were performed with QIIME, LEfSe, and R.

Results

A total of 178 species, belonging to 62 genera and 8 phyla were identified. Genera Strepto

SUBMITTER: Halboub E 

PROVIDER: S-EPMC7346439 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature