RAP1-RAC1 Signaling Has an Important Role in Adhesion and Migration in HNSCC.

ABSTRACT: Cell-cell adhesion is a key mechanism to control tissue integrity and migration. In head and neck squamous cell carcinoma (HNSCC), cell migration facilitates distant metastases and is correlated with poor prognosis. RAP1, a ras-like protein, has an important role in the progression of HNSCC. RAC1 is an integrin-linked, ras-like protein that promotes cell migration. Here we show that loss of cell-cell adhesion is correlated with inactivation of RAP1 confirmed by 2 different biochemical approaches. RAP1 activation is required for cell-matrix adhesion confirmed by adhesion to fibronectin-coated plates with cells that have biochemically activated RAP1. This effect is reversed when RAP1 is inactivated. In addition, RAP1_GTP-mediated adhesion is only facilitated through α5β1 integrin c

SUBMITTER: Liu M 

PROVIDER: S-EPMC7346744 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature