MitoPlex: A targeted multiple reaction monitoring assay for quantification of a curated set of mitochondrial proteins.

ABSTRACT: Mitochondria are the major source of cellular energy (ATP), as well as critical mediators of widespread functions such as cellular redox balance, apoptosis, and metabolic flux. The organelles play an especially important role in the maintenance of cardiac homeostasis; their inability to generate ATP following impairment due to ischemic damage has been directly linked to organ failure. Methods to quantify mitochondrial content are limited to low throughput immunoassays, measurement of mitochondrial DNA, or relative quantification by untargeted mass spectrometry. Here, we present a high throughput, reproducible and quantitative mass spectrometry multiple reaction monitoring based assay of 37 proteins critical to central carbon chain metabolism and overall mitochondrial function termed 'MitoP

SUBMITTER: Stotland AB 

PROVIDER: S-EPMC7347090 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature