All three mammalian MutL complexes are required for repeat expansion in a mouse cell model of the Fragile X-related disorders.

ABSTRACT: Expansion of a CGG-repeat tract in the 5' untranslated region of the FMR1 gene causes the fragile X-related disorders (FXDs; aka the FMR1 disorders). The expansion mechanism is likely shared by the 35+ other diseases resulting from expansion of a disease-specific microsatellite, but many steps in this process are unknown. We have shown previously that expansion is dependent upon functional mismatch repair proteins, including an absolute requirement for MutLγ, one of the three MutL heterodimeric complexes found in mammalian cells. We demonstrate here that both MutLα and MutLβ, the two other MutL complexes present in mammalian cells, are also required for most, if not all, expansions in a mouse embryonic stem cell model of the FXDs. A role for MutLα and MutLβ is consistent with human GWA stu

SUBMITTER: Miller CJ 

PROVIDER: S-EPMC7347238 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature