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High-Throughput Cryo-EM Enabled by User-Free Preprocessing Routines.

ABSTRACT: Single-particle cryoelectron microscopy (cryo-EM) continues to grow into a mainstream structural biology technique. Recent developments in data collection strategies alongside new sample preparation devices herald a future where users will collect multiple datasets per microscope session. To make cryo-EM data processing more automatic and user-friendly, we have developed an automatic pipeline for cryo-EM data preprocessing and assessment using a combination of deep-learning and image-analysis tools. We have verified the performance of this pipeline on a number of datasets and extended its scope to include sample screening by the user-free assessment of the qualities of a series of datasets under different conditions. We propose that our workflow provides a decision-free solution for cryo-EM, making data preprocessing more generalized and robust in the high-throughput era as well as more convenient for users from a range of backgrounds.

SUBMITTER: Li Y

PROVIDER: S-EPMC7347462 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature

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High-Throughput Cryo-EM Enabled by User-Free Preprocessing Routines.

Li Yilai Y Cash Jennifer N JN Tesmer John J G JJG Cianfrocco Michael A MA

Structure (London, England : 1993) 20200414 7

Single-particle cryoelectron microscopy (cryo-EM) continues to grow into a mainstream structural biology technique. Recent developments in data collection strategies alongside new sample preparation devices herald a future where users will collect multiple datasets per microscope session. To make cryo-EM data processing more automatic and user-friendly, we have developed an automatic pipeline for cryo-EM data preprocessing and assessment using a combination of deep-learning and image-analysis to ...[more]

PMID: 32294468

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