Dnmt1 function is required to maintain retinal stem cells within the ciliary marginal zone of the zebrafish eye.

ABSTRACT: The ciliary marginal zone (CMZ) of the zebrafish retina contains a population of actively proliferating resident stem cells, which generate retinal neurons throughout life. The maintenance methyltransferase, dnmt1, is expressed within the CMZ. Loss of dnmt1 function results in gene misregulation and cell death in a variety of developmental contexts, however, its role in retinal stem cell (RSC) maintenance is currently unknown. Here, we demonstrate that zebrafish dnmt1^s872 mutants possess severe defects in RSC maintenance within the CMZ. Using a combination of immunohistochemistry, in situ hybridization, and a transgenic reporter assay, our results demonstrate a requirement for dnmt1 activity in the regulation of RSC proliferation, gene expression and in the repression of endogen

SUBMITTER: Angileri KM 

PROVIDER: S-EPMC7347529 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature