Glucosamine potentiates the differentiation of adipose-derived stem cells into glucose-responsive insulin-producing cells.

ABSTRACT:

Background

Islet transplantation might be a logical strategy to restore insulin secretion for the treatment of diabetes, however, the scarcity of donors poses an obstacle for such a treatment. As an alternative islet source, differentiation of stem cells into insulin-producing cells (IPCs) has been tried. Many protocols have been developed to improve the efficiency of differentiation of stem cells into IPCs. In this study, we investigated whether glucosamine supplementation during differentiation of human adipose-derived stem cells (hADSCs) into IPCs can improve the insulin secretory function.

Methods

Glucosamine was added to the original differentiation medium at different stages of differentiation of hADSCs into IPCs for 12 days and insulin secretion was analyzed.

Resul

SUBMITTER: Hong Y 

PROVIDER: S-EPMC7347784 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature